Bis(trimethylsilyl)trifluoroacetamide: A Powerful Derivatizing Agent for Gas Chromatography
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Bis(trimethylsilyl)trifluoroacetamide: A Powerful Derivatizing Agent for Gas Chromatography
Bis(trimethylsilyl)trifluoroacetamide (BSTFA) is a popular derivatizing agent used in gas chromatography (GC) analysis of various compounds, including fatty acids, steroids, and drugs. It is a clear, colorless liquid with a pungent odor and a molecular weight of 341.5 g/mol. BSTFA is produced by INNO Specialty Chemicals, a leading manufacturer and supplier of high-quality specialty chemicals based in China.
BSTFA is a highly reactive silylating agent that can react with a wide range of functional groups, such as hydroxyl, carboxyl, and amino groups, to form volatile and stable derivatives. This silylation reaction is essential for GC analysis because it improves the volatility, thermal stability, and chromatographic behavior of the analytes. The silylated derivatives are less polar, more volatile, and have longer retention times than the original compounds, which enhances their separation, detection, and quantification in GC.
The silylation reaction with BSTFA involves the substitution of the labile hydrogen atoms of the functional groups with trimethylsilyl (TMS) groups, which are more stable and less polar. The reaction is typically carried out in the presence of a catalyst, such as N-methylimidazole (NMI), and a solvent, such as pyridine or hexane, at elevated temperatures (usually 60-70°C) for several hours. The reaction progress can be monitored by GC or thin-layer chromatography (TLC) analysis.
BSTFA is a versatile and efficient derivatizing agent that can be used for various types of samples and applications. It is particularly useful for derivatizing compounds that are sensitive to other silylating agents, such as trimethylchlorosilane (TMCS) or N,O-bis(trimethylsilyl)acetamide (BSA). BSTFA is also preferred over other silylating agents because it produces fewer artifacts, such as side reactions or rearrangements, and gives cleaner chromatograms.
BSTFA has been widely used in GC analysis of various compounds, such as fatty acids, steroids, and drugs. For example, BSTFA can derivatize free fatty acids to their TMS esters, which are more volatile and have sharper peaks than the original acids. BSTFA can also derivatize steroids, such as testosterone and estradiol, to their TMS ethers, which are more stable and have longer retention times than the original steroids. Moreover, BSTFA can derivatize drugs, such as amphetamines and opioids, to their TMS derivatives, which are more volatile and have better sensitivity and selectivity than the original drugs.
BSTFA is a reliable and cost-effective reagent for GC analysis, and its quality and purity are critical for obtaining accurate and reproducible results. INNO Specialty Chemicals is committed to providing high-quality and consistent BSTFA products to meet the needs of its customers. The company's BSTFA products are characterized by their high purity (>99%), low water content (<0.01%), and low levels of impurities, such as trimethylsilyl trifluoroacetamide (TMSA), trimethylsilyl acetate (TMSA), and N,O-bis(trimethylsilyl)trifluoroacetamide (BSFTA).
In conclusion, Bis(trimethylsilyl)trifluoroacetamide is a powerful derivatizing agent for gas chromatography that can improve the volatility, thermal stability, and chromatographic behavior of various compounds. It is a versatile and efficient reagent that can be used for various types of samples and applications. INNO Specialty Chemicals is a reliable manufacturer and supplier of high-quality BSTFA products that meet the demanding requirements of GC analysis. BSTFA is an essential tool for GC analysts who want to achieve accurate and reliable results in their research and analysis.
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